XE-991 facilitates the induction of LTP by theta burst pairing.

<p>A) Diagram of theta burst pairing (TBP) protocol. Top, voltage trace of TBP protocol recorded at the soma of a CA1 pyramidal cell. Middle and bottom traces illustrate the timing of inputs to the stimulating and recording electrodes evoking EPSPs and somatic action potentials respectively. B) TBP does not induce LTP under control conditions. Coincident TBP of subthreshold EPSPs and somatic action potentials induced no change in EPSC amplitude in the test (black circles) or control (white circles) pathways. The arrow indicates the timing of the TBP protocol. Example voltage traces show the initial burst of 5 coincident EPSPs and action potentials and a single test burst of 5 subthreshold EPSPs. Example current traces from a single experiment illustrating the mean EPSC response during the baseline (1) and at 30–35 minutes (2) in the test and control pathways. C) TBP does induce LTP in the presence of the Kv7 channel inhibitor XE-991. In the presence of XE-991 (10 µM), coincident TBP of subthreshold EPSPs and somatic action potentials induced pathway-specific LTP.