Vaccination with irradiated B16-mBD2 inhibits the growth of implanted tumors and prolonged the survival of mice.

<p>(A, B) The preventive effect of B16-mBD2 vaccination on the development of tumors. Groups of C57BL/6 mice were injected with saline or vaccinated with irradiated parental B16 cells, B16-p cells, or B16-mBD2 cells, respectively, and the mice were inoculated s.c with B16 tumor cells one week later. The growth of tumors in different groups of mice were monitored up to 20 days post inoculation (n = 8 per group), and their survival was monitored up to 150 day post inoculation (n = 8 per group). Data are a representative of three separate experiments and expressed as mean ± SD of the tumor volumes of each group of mice. (C, D) The therapeutic effect of vaccination with irradiated B16-mBD2 on the progression of implanted tumors. Groups of C57BL/6 mice were inoculated s.c with B16 cells, and then injected with saline or treated with irradiated parental B16 cells, B16-p cells, or B16-mBD2 cells for four times, respectively. The growth of tumors were monitored (n = 8 per group), and their survival were monitored up to 150 day post-treatment (n = 8 per group). Data are a representative of three independent experiments and expressed as mean ± SD of the tumor volumes of each group of mice.