<i>In vitro</i> integration in buffers of varying MgCl₂ concentration.

<p>(A) Electrophoresis of plasmid DNA with (upper) or without (lower) the target sequence DNA. Supercoiled DNA was electrophoresed at 0 min, at 30 min, and at 60 min after incubation. Arrows and arrowheads indicate fragments corresponding to peaks shown in (B). (B) Electropherogram of the plasmid with the target sequence and the plasmid alone at incubation for 0 min and 30 min. (C) Graph showing the relative area of the electrophoretic signal of supercoiled plasmid DNA with (red) or without (blue) target <i>Stat5a</i> DNA at 60 min after incubation in buffer containing various concentrations of MgCl₂ (unit mM, n = 6; mean ± s.d.). (D) Graph showing the total number of integration sites within the target <i>Stat5a</i> DNA (red) and the number of integrations at nucleotide 1130 (blue) at 60 min after incubation in buffer containing various concentrations of MgCl₂ (unit mM, n = 6; mean ± s.d.). (E) Sample photo of a secondary structure when using a buffer containing 60 mM and 30 mM of manganese dichloride (mM). Supercoiled DNA (in the upper photo) and globular DNA (in the lower photo) are displayed.