Interaction of αB-wt and αA-wt or its mutants by <i>in situ</i> FRET.

<p>Examples of the acceptor photobleaching method for determining the FRET efficiency. In this example, CFPαBwt (donor) was co-expressed with either YFPαA-wt or YFPαA-R2116C (acceptor). The acceptor fluorescence was bleached by high intensity argon laser light. This resulted in an increase in donor fluorescence intensity and a decrease in acceptor fluorescence. The actual FRET efficiency was measured from at least 50 regions of interest (ROI) in the cell images obtained in three independent experiments for each pair.