Gu-4 inhibited the adhesion and subsequent transendothelial migration of THP-1 cells.

<p>HUVECs and calcein AM-labeled THP-1 cells were pretreated as described in Material and methods and subjected to adhesion and migration assays. A: In adhesion assay, THP-1 cells with LPS stimulation showed enhanced adhesion to HUVECs, whereas THP-1 cells treated with Gu-4 or CD11b antibody, but not β-lactose, after LPS stimulation showed no significant increase of adhesion compared with control cells. The experiments were performed in triplicate. *: p<0.05 <i>vs.</i> LPS (−) control. #: p<0.05 <i>vs.</i> LPS (+) control. B: In migration assay, LPS-stimulated THP-1 cells were subjected to treatments of Gu-4, β-lactose, CD11b antibody, or saline, and then seeded into inserts with HUVECs monolayer on the outer side of the membrane. The relative amount of migrated cells in the lower chamber were determined by measurement of fluorescence intensity. Both Gu-4 and CD11b antibody significantly reduced the migration of THP-1 cells across HUVECs monolayer. The experiments were performed in triplicate. *: p<0.05 <i>vs.</i> saline.