Localization of TG2 activity in the small intestine of poly(I∶C)-treated mice.

<p>Mice were treated with 30 mg/kg poly(I∶C), and samples were collected from the duodenum (a–d), jejunum (e–h), and ileum (i–l). Active TG2 was detected by exposing cross-sections to Alexa fluor 555-conjugated (b, f, j). The total amount of TG2 protein in these sections was visualized by co-staining with polyclonal anti-TG2 antibody, followed by an Alexa fluor 488-conjugated secondary antibody (c, g, k). Nuclei were stained with DAPI (a, e, i). Overlays are shown in the right column (d, h, l). Scale bar represents 300 µm and applies to all panels. At least 4 animals were tested; one representative section is shown for each intestinal segment.