Involvement of VEGFR2 in EC barrier dysfunction induced by high OxPAPC concentration.

<p><b>A</b> – siRNA-induced VEGFR2 depletion in HAEC and HPAEC at mRNA and protein levels was compared to treatment with non-specific RNA. Membrane re-probing with β-tubulin antibodies was used as normalization control. <b>B</b> – HPAEC grown on gold microelectrodes for TER measurements were transfected with siRNA specific to VEGFR2 (100 nM). Control cells were transfected with non-specific RNA. After 72 hrs of transfection, cells were stimulated with OxPAPC (75 µg/ml, shown by arrow) or vehicle, and permeability changes were monitored over 6 hrs. <b>C</b> - Bar graphs depict TER changes measured after 4 hrs of Ox-PAPC stimulation marked by dotted line in panel B; n = 4–6 per condition; *p<0.05.