<p>Mean percentages (± standard deviation) of isolated cytokeratin (CK)+ CD45− cells from jejunum either after DTT, EDTA or collagenase treatment with or without prior DTT wash (as discussed in details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030247#pone-0030247-g001" target="_blank">Figs. 1</a> & <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030247#pone-0030247-g002" target="_blank">2</a>) are shown from uninfected normal healthy rhesus macaques (n = 5). Note that there was increased yield of ECs either by DTT or EDTA only treatment compared to other protocols followed in this experiment. Statistical significant differences between each group of cells are shown. * Indicates signific...
Studies on immune cells derived from the human intestine are needed to understand the pathogenesis o...
<p>(<b>A</b>) The magnitude of the SIV-specific IFN-γ T cell response in the gut was determined by E...
<p>(<b>A</b>) Left contour plot shows the IL-10R positive cells in the gated region from a normal he...
<p>Representative dot plots of total cells from (<b>A</b>) DTT wash (<a href="http://www.plosone.org...
<p>Representative dot plots of total cells from (<b>A</b>) upper layer of percoll density gradient i...
<p>(<b>A</b>) Representative expression of cytokeratin (CK; epithelial cell marker) and CD45 (leukoc...
<p>Note that this protocol explains cell isolation procedures with initial EDTA treatment. No prior ...
Aim: The epithelial layer within the colon represents a physical barrier between the luminal content...
<p>Western blot for intra- and extra-cellular domains of E-cadherin of IEC-18 cells without a mucin ...
<p>(<b>A</b>) <b>Comparison of the percentage of CD3<sup>+</sup>CD4<sup>+</sup>-cells in collagenate...
<p>A. Flow cytometry plots and frequency of CD45+ cells of live cells. B. Frequency of CD3, CD20, CD...
<p>A) FACS analysis of T cell contamination. Positive selection of IEC was performed using CD326 Mic...
<p><b>(A)</b> Bar graph showing relative absorbance values from an ELISA with immobilized anti-Le<su...
Background and MethodsCell isolation from macaque tissues involves laborious enzymatic digestion. Th...
<p><b>A.</b> Phase contrast or immunofluorescence images of DKAT cells cultured in either MEGM (uppe...
Studies on immune cells derived from the human intestine are needed to understand the pathogenesis o...
<p>(<b>A</b>) The magnitude of the SIV-specific IFN-γ T cell response in the gut was determined by E...
<p>(<b>A</b>) Left contour plot shows the IL-10R positive cells in the gated region from a normal he...
<p>Representative dot plots of total cells from (<b>A</b>) DTT wash (<a href="http://www.plosone.org...
<p>Representative dot plots of total cells from (<b>A</b>) upper layer of percoll density gradient i...
<p>(<b>A</b>) Representative expression of cytokeratin (CK; epithelial cell marker) and CD45 (leukoc...
<p>Note that this protocol explains cell isolation procedures with initial EDTA treatment. No prior ...
Aim: The epithelial layer within the colon represents a physical barrier between the luminal content...
<p>Western blot for intra- and extra-cellular domains of E-cadherin of IEC-18 cells without a mucin ...
<p>(<b>A</b>) <b>Comparison of the percentage of CD3<sup>+</sup>CD4<sup>+</sup>-cells in collagenate...
<p>A. Flow cytometry plots and frequency of CD45+ cells of live cells. B. Frequency of CD3, CD20, CD...
<p>A) FACS analysis of T cell contamination. Positive selection of IEC was performed using CD326 Mic...
<p><b>(A)</b> Bar graph showing relative absorbance values from an ELISA with immobilized anti-Le<su...
Background and MethodsCell isolation from macaque tissues involves laborious enzymatic digestion. Th...
<p><b>A.</b> Phase contrast or immunofluorescence images of DKAT cells cultured in either MEGM (uppe...
Studies on immune cells derived from the human intestine are needed to understand the pathogenesis o...
<p>(<b>A</b>) The magnitude of the SIV-specific IFN-γ T cell response in the gut was determined by E...
<p>(<b>A</b>) Left contour plot shows the IL-10R positive cells in the gated region from a normal he...