Phenotyping colon and jejunum epithelial cells (ECs) by confocal microscopy.

<p>Essentially all colon (<b>A–E</b>) ECs (cytokeratin positive) were negative for (<b>A</b>) Ham56 (macrophage marker), and (<b>B</b>) CD11c (dendritic cell marker). Very few colon ECs were double positive for (<b>C</b>) both CD45 (leukocyte marker) and cytokeratin expression. A major population of colon ECs was positive for (<b>D</b>) HLA-DR expression. However, very few colon ECs were positive for (<b>E</b>) IL-10R expression which was evident in apical regions. Jejunum ECs were also negative for (<b>F</b>) CD54 (ICAM-1, cell adhesion marker). White arrow denotes the presence of double positive cells (cytokeratin and CD45/HLA-DR/IL-10R) for the specified sample and fluorochrome.