Effect of UO on post-ischemic polymorphonuclear leukocyte (PMN) infiltration in different strains.

<p>At different time points (0 d, 2 d, 4 d, 7 d) after release of UO, animals were subjected to renal IR, and then the left kidneys were harvested and immunostained for MPO activity. A, representative renal sections from mice of UO 2 d group and their non-UO controls (original magnification ×200). Sections from HIF-1α^−/−, HIF-2α^−/− and DKO mice in non-IR groups had no PMN infiltration and were not shown. B, PMN infiltration was scored on a scale of 1–4. The results of different strains at all tested time points were presented. All mice that were subjected to non-IR sham operation had no PMN infiltration and the results were not shown. Data were expressed as mean ± SD from 6–8 animals per genotype. *, p<0.05 versus WT mice that were treated with the same procedures.