Astrocyte activation in LGN layers receiving input from a glaucomatous eye.

<p>(A) Representative photographs of GFAP immunoreactivity in LGN of the contralateral and ipsilateral sides are shown for each experimental glaucoma stage. Each experimental glaucoma stage was classified by the glaucoma severity based on the extent of axonal loss as mild (0–20%), moderate (20–50%), and advanced (50–100%). Layer 1 on the contralateral side and layer 2 on the ipsilateral side receive their innervations from the glaucomatous (left) eye (shown in bold frame), whereas layer 1 on the ipsilateral side and layer 2 on the contralateral side receive their retinal inputs from the non-treated fellow (right) eye. L1 and L2: layer 1 and 2. Scale bars indicate 1000 µm in whole LGN image and 50 µm in enlarged image. (B) The GFAP-immunoreactive area on each section was measured, and averaged for each LGN as mean ± S.E.M. at each M-(layers 1 and 2) and the P-(layers 3–6) layers.