NMII induced apoptosis depends on <i>C. burnetii</i> replication.

<p>NMII infected THP-1 cells were treated with 10 µg/ml rifampin up to 72 h post infection. Panel 8A, quantification of <i>C. burnetii com1</i> gene copies by real-time PCR. Panel 8B, Western blot of rifampin inhibition in NMII infected THP-1 cells. Lane 1, normal cells; Lane 2, staurosporine treated positive control cells; Lane 3, NMII (MOI 5) infected cells at 24 h post infection; Lane 4, NMII (MOI 50) infected cells at 24 h post infection; Lane 5, NMII (MOI 5) infected cells with rifampin treatment at 24 h post infection; Lane 6, NMII (MOI 50) infected cells with rifampin treatment at 24 h post infection; Lane 7, NMII (MOI 5) infected cells at 48 h post infection; lane 8, NMII (MOI 50) infected cells at 48 h post infection; Lane 9, NMII (MOI 5) infected cells with rifampin at 48 h post infection; Lane 10, NMII (MOI 50) infected cells with rifampin treatment at 48 h post infection.