NLRP12 deficiency does not influence the development of acute allergic airway inflammation.

<p><b>A</b>) Schematic illustrating OVA mediated acute allergic airway inflammation with an alum adjuvant. <b>B</b>) Mice immunized with OVA/alum and challenged with OVA (OVA/OVA) demonstrated a significant increase in BALF cellularity compared to wild type and <i>Nlrp12^−/−</i> mice immunized with OVA/alum but challenged with saline (OVA/Saline). <b>C</b>) Differential staining of the BALF cellularity revealed that mice immunized and challenged with OVA demonstrated a significant increase in airway leukocyte populations, which were predominately eosinophilic. <b>D</b>) A significant increase in local (BALF) and systemic (serum) IL-13 was observed in all OVA immunized and challenged animals regardless of genotype. OVA/Saline, n = 6; <i>Nlrp12^−/−</i>, n = 12; wild type, n = 15. <b>E</b>) A significant increase in OVA specific IgE was detected in serum collected from both wild type and <i>Nlrp12^−/−</i> mice following OVA immunization and challenge. <b>F</b>) Central airway resistance (Rn) and tissue damping (G) in response to methacholine (MCh) was evaluated in wild type and <i>Nlrp12^−/−</i> mice following OVA challenge. Mock (PBS), n = 4; Wild Type, n = 8; <i>Nlrp12^−/−</i>, n = 5.