CYP3A enzymatic activity analysis.

<p>Five adult male miR-shRNA1 transgenic mice, unrelated miR-shRNA transgenic mice and wild-type mice were subjected to CYP3A enzymatic activity analysis in parallel. The ages of used mice were 8–10 weeks. Analysis was performed using liver microsome systems prepared from each group, and testosterone was added into the systems up to a defined concentration as the probe substrate. The concentration of the oxidative metabolite of probe substrate (6β-hydroxyl-testosterone) was detected by HPLC analysis. Wild-type: wild-type mice of FVBN strain; unrelated miR-shRNA: transgenic mice expressing a miR-shRNA targeting luciferase; miR-shRNA1: transgenic mice expressing the miR-shRNA1 targeting CYP3A. * indicates statistic significance.