Presence of p14 in caprine sperm cells and in epididymal epithelial tissue.

<p>Rabbit polyclonal antisera were raised against p14 protein (antigen), purified by affinity purification procedure and was used for Western blotting. (<b>A</b>) Western blot analysis of p14 in total tissue extracts from caput (Cap), corpus (Cor) and cauda (Cau) of the epididymis and vas deferens (Vd). Blot was probed with monoclonal antibody against actin to assess protein loading. (<b>B</b>) Western blot analysis showing p14 in total protein extracts of sperm from caput (Cap), corpus (Cor) and cauda (Cau) regions of the epididymis and vas deferens (Vd). Actin was used as loading control. (<b>C</b>) The immunohistochemical staining to show the expression pattern of p14 in the epithelial cells and lumen of the caput, corpus and cauda epididymis. Preimmune serum at the same condition showed no immunoreactivity. All the analysis was performed four times.