NLRP12 deficiency did not influence mucus production or collagen deposition following OVA sensitization and challenge.

<p><b>A</b>) Mucus production was assessed following treatment with the Alcian blue/periodic acid-Schiffs reaction (AB/PAS) (20× original magnification). <b>B</b>) The volume of AB/PAS-stained mucosubstance per square millimeter of basal lamina (Vs) was determined. A significant increase in mucus was observed in the OVA/OVA mice; however, no significant differences were observed between the <i>Nlrp12^−/−</i> and wild type mice. <b>C</b>) Masson's trichrome staining was used to assess collagen deposition in the lungs. No significant differences were observed for collagen deposition in the acute allergic airway inflammation model, regardless of genotype or treatment. OVA/Saline, n = 6; <i>Nlrp12^−/−</i>, n = 12; wild type, n = 15.