Effect of capacitation on the distribution of sperm p14.

<p>(<b>A</b>) Indirect immunofluorescence of anti-p14 labeled protein in live capacitated caprine spermatozoa. Upper panel showing the fluorescent and corresponding phase contrast micrographs of noncapacitated sperm (control). Lower panels showing the same in sperm capacitated for 3 hrs. Three different patterns of staining were observed. (<b>B</b>) Indirect immunofluorescence of anti-p14-labeled protein in fixed/permeabilized caprine sperm. Upper panels showing the fluorescence and corresponding phase contrast pattern of noncapacitated sperm. Lower panels correspond to the same but for 3 hrs capacitated spermatozoa. Two different patterns of immune-fluorescence were observed. The calculated percentages given as the mean ± SEM of at least four (n = 4) different experiments.