Detection and quantification of XY cells from donor origin in the endometria of BMT recipients.

<p>(A&B) Human endometrial sections from 2 different patients, who underwent BMT, showing a positive FISH signal corresponding to chromosome Y (green signal) and chromosome X (orange signal) along both the epithelial and stromal compartments. Presence of donor-derived cells (chromosome Y-positive cells) in the endometrium is indicated by arrows. Lower panels containing control slides: (A) endometrium as a positive control for the chromosome X signal and (B) fetal testes as a positive control for the chromosome XY signal. (C) Table summarizing the characteristics of the recipients, the percentages of XY-positive cells obtained in the whole endometrium, plus the epithelial and stromal fractions. All the images were acquired using a 63×/1.6× oil immersion objective.