<i>Bone marrow derived CD11b+CCR2+ cells are essential to support tumor development and angiogenesis.</i>

<p>(<b>A</b>) CD11b+ BMD cells from <i>cx₃cr1</i>^gfp CCR2+ CD45.1 mice were purified (left panel), analyzed fro the relative mummer of GFP+ cells (right panel) and transferred to CCR2−/− mice bearing CCR2+ tumor (<b>B</b>) shows imaging (IVIS) of a representative mouse as recorded using a GFP filter. (<b>C</b>) Imaging (IVIS) of the primary tumor on day 60, as recorded by the IVIS camera using – luciferin filter (recording luciferase activity of the cancer cells) as follows: CCR2+/+ C57BL/6 mice (WT) (a), CCR2−/− mice (b), CCR2−/− transplanted with BM of WT mice(c) and CCR2−/− transplanted with BM of CCR2−/−mice. All photos show a representative mouse per group (1 of 6 mice). (<b>D</b>) The computerized CCCD analysis of six mice per group. Results are shown as total flux (p/s ×10⁴) ±SE. * Indicates p<0.001 (<b>E</b>) Histological, Immunohistochemical and immunofluorescence analyses of primary tumors from CCR2^+/+ C57BL/6 mice (WT), CCR2^−/− C57BL/6 mice and BM transplanted CCR2^−/− mice. Panel a–c show H&E staining, d–f show anti -PCNA staining, g–i show anti F4/80, j–l show anti VEGF and m–o show anti CD31.