CD1b⁺ L-DCs are migratory mature DC capable of endocytic and phagocytic activities.

<p>After labelling lymph cells with anti- CD1b mAb (A), CD1b⁺ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from 3 sheep (B). Plots of different markers expressed by CD1b⁺ L-DCs are shown (C). May-Grünwald Giemsa staining of sorted and cytocentrifugated CD1b⁺ L-DCs (D). Expression of mRNA for CCR7, CD103 and DC-SIGN by sorted CD1b⁺ L-DCs (1, 3, 5 respectively) and in cDNA control (2, 4, 6 respectively) (E). Uptake of FITC-ovalbumin (FITC-OVA) or GFP-<i>Salmonella</i> by CD1b⁺ L- DCs. Lymph cells (1×10⁶ cells) were incubated with FITC-OVA for 1h at 4°C or 37°C, or with GFP-<i>Salmonella</i> for 30 min at 37°C, followed by CD1b labelling. CD1b⁺ L- DC subset was gated on lymph cells and FITC-OVA fluorescence shown on dot plots F and G. Total L-APC were gated on SSC/FSC and the cell population negative for lymphocyte markers. Quadrants were then defined on the CD1b⁺ and CD1b^- subsets and GFP-<i>Salmonella</i> fluorescence shown on dot plot H.