Size of NP oligomers in the presence of RNA monitored by Dynamic Light Scattering.

<p><b>A</b> - Size distribution of monomeric wt NP (5 µM) alone (black, 6.8 nm, 93%, 5.0 µ, 7%) and 1 hour (dotted blue, 13.8 nm 91%, 760 nm 9%) or 3 hours after addition of 1.8 µM RNA (16.3 nm, 100%); <b>B</b>- Size distribution of monomeric R361A (5 µM) alone (black, 7.8 nm, 100%) and 4 hours after addition of 1.8 µM RNA (dashed green, 9.85 nm, 75%, 279 nm, 25%) <b>C</b>: Comparison of the oligomerization kinetics of R361A (violet squares) and R361A-E80A-E81A (green stars) (10 µM) after addition of RNA (3 µM). Note the large difference in the final size of the protein-RNA oligomers being 10±1 nm and 16±1 nm for R361A and R361A-E80A-E81A, the latter resembling the size of oligomeric NP-RNA complexes.