Figure 3

<p>(a) Intrinsic fluorescence spectrum of HspB2. The excitation wavelength was 295 nm. The excitation and the emission band passes were set at 2.5 nm. The concentration of the proteins was 0.2 mg/ml. Fluorescence intensity is represented in arbitrary units. (b) Lehrer plot of tryptophan fluorescence quenching of HspB2 by KI. Fluorescence was monitored after sequential addition of KI to a solution containing 0.2 mg/ml HspB2. Insert shows the decrease in fluorescence intensity upon sequential addition of KI.