Activation of MAP kinases in H1N1pdm-infected 3D/4 cells.

<p>Swine macrophages were infected with H1N1pdm virus and cell lysates were prepared at various time points after infection, and subsequently subjected to SDS-PAGE and western blot analysis to show phosphorylation and activation of MAP kinases and NFκB. Background phosphorylation of 3D/4 cells was also measured over the time without infection. <b>A.</b> Phosphorylation of swine ERK1/2. Proteins were analyzed with anti-ERK1/2 and anti-phospho-ERK1/2 antibodies. <b>B.</b> Phosphorylation of swine JNK1/2. Proteins were analyzed with anti-JNK1/2 and anti-phospho-JNK1/2 antibodies. C. Background phosphorylation of ERK1/2 and JNK1/2. After 16 hr culture, uninfected 3D/4 cells were taken at various time points for western blot analyses to measure background phosphorylation.