HIV-1 particles produced by Dlg1-depleted cells accumulated higher total HIV-1 DNA amounts during the first hours of infection and have increased cholesterol content.

<p><b>A. Quantification of</b> t<b>otal viral DNA.</b> Total viral DNA was quantified by quantitative PCR in Jurkat T cells 6 h after infection with NL4.3 or LAI.2 HIV-1 particles produced by Dlg1+ and Dlg1- cells. The results are expressed as mean NRQ (normalized RQ described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030130#s2" target="_blank">materials and methods</a>). Values are from one representative experiment performed in triplicate for infection and in triplicate for qPCR. P = 0.001. <b>B. Viral cholesterol content of HIV-1 particles produced by 293T cells.</b> Dlg1- 293T cells were obtained by siRNA transfection. The cholesterol content of LAI.2 HIV-1 produced by Dlg1+ or Dlg1- 293T cells was determined. The data are the means of three independent experiments performed in duplicate. For each experiment the values were normalized to the value obtained with one of the duplicates of viruses from Dlg1+ cells taken as 100%. P = 0.0026. <b>C. Viral cholesterol content of HIV-1 particles produced by Jurkat T cells.</b> Cholesterol content of LAI.2 HIV-1 produced by Dlg1+ or Dlg1- Jurkat T cells was determined. The data are the means of one experiment performed in duplicate. The values were normalized to the value obtained for one of the duplicates of viruses from Dlg1+ cells taken as 100%. Error bars represent SEM. **, P<0.01.