EV71 infection is impacted by clathrin specific inhibitors.

<p>(A) 3T3-SCARB2 cells were pretreated with different doses of CPZ for one hour prior to the addition of MOI = 0.04 of EV71. (B) 50 or 100 pmoles of siRNA specific to clathrin or 100 pmoles of control siRNA (MOCK) were transfected to 3T3-SCARB2 cells as following the treatment protocol as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030507#pone-0030507-g004" target="_blank">Figure 4</a> described above. After 24 or 48 hours of transfection, cells were following lysed and detected the content of clathrin light chain B (CLTB) by Western blot using anti-clathrin antibody. Relative expression of siRNA-targeting genes shown in the top panel represented the relative CLTB protein levels of the cells transfected with specific siRNA, compared to the expression in 48 hours transfection of the cells with 100 pmoles control siRNA (Mock) as 1.0 was shown. Lysates were also prepared after 24 or 48 hours of EV71 infection and subjected to analyze the expression of synthetic EV71 capsid protein by western blot using MAB979 antibody. The result was shown in the lower panel. Cellular β-actin was detected by blotting the same membrane with monoclonal anti-β-actin antibody. Data represent one of two independent experiments.