Titration of individual CPC markers before multicolour flow cytometry.

<p>Data are presented as geometric means with 95% confidence intervals. When without dilution, the volume of each antibody used for staining 100 µ0 of blood is: 10 µ0 of CD133-APC (50 CD133), 5 µL of CD34-PECy7 (50 µg/mL), 20 µL of KDR-FITC (50 µg/mL), 20 µL of CD164-PE (50 µg/mL), 5 µL of CD117-PE (50 µg/mL), and 20 µL of CD164-PE (25 µg/mL), respectively. The green bars show the dilution ratio of individual antibodies as recommended by vendors. The purple bars show the optimal dilution ratio of some antibodies according to our titration tests.