Effect of small interfering (si) and scrambler (sc) RNA against Nrf2 on oxPAPC-dependent expression of GCLC and HO-1 in human umbilical vein endothelial cells.

<p>mRNA (a) was analyzed by quantitative Real-Time PCR. Normalized gene expression levels were given as the ratio between the mean value for the target gene and that for the beta-actin in each sample. Figure shows a representative Western blot analysis for GCLC and HO-1 and the average quantification obtained by densitometric analysis of all the samples (b). Data on Western blot analysis are expressed as the density ratio of target to control (beta-actin) in arbitrary units ×10. Results are the mean±SD of measurements performed in triplicate in four different occasions. *P<0.01 versus control; †P<0.01 versus scRNA; #P<0.01 versus oxPAPC+scRNA.