Effect of lysosomal inhibitors or ASM gene silencing on HG-induced increase of lamp1 protein quantity on cellular membrane.

<p>The proteins on cellular membrane were first isolated and then the lamp1 expression was detected by Western blot. A: representative band of lamp1 expression in cell incubated by normal glucose (Ctrl) or high glucose for 12 h (HG) with or without the pretreatment by vacuolin-1(10 µmol/l), bafilomycin A1 (100 nmol/l), cPLA 2α (20 µmol/l) for 1 h, or after scramble sRNA or ASM siRNA transfection. B: summarized data of lamp 1 protein quantity normalized by β-actin in different groups. Values are means±SE; n = 4 batches of cells. * P<0.05 vs. Ctrl.