Detection of the rabbit FcRn in Western blot.

<p>This Western blot shows that the chicken antibody strongly and specifically reacted with the soluble recombinant bovine FcRn which was used for immunization (its estimated molecular weight is 30 kDa), a ∼40-kDa band that is consistent with the known molecular weight of the bovine FcRn α-chain in the protein extract from a bovine FcRn stably transfected cell line (B4) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028869#pone.0028869-Kacskovics4" target="_blank">[46]</a> which strongly expresses the functional form of the bovine FcRn and a ∼40-kDa band that is consistent with the calculated molecular weight of the rabbit FcRn α-chain in the protein extract from the rabbit yolk sac from 24 dpc fetuses.