Association of virus-specific IFN-γ and serum neutralising antibody responses with viraemia.

<p>Pigs were vaccinated with C-strain CSFV on days -5, -3 or -1 and were then challenged, together with groups of unvaccinated pigs, with UK2000/7.1 or CBR93 CSFV isolates. According to clinical outcome following challenge, the day -1 vaccinated pigs were separated into two groups; (recov) animals that experienced mild signs followed by recovery and (euth) animals that developed severe signs necessitating euthanasia. (A) Peripheral blood leukocyte (PBL) IFN-γ responses were measured by ELISA following <i>in vitro</i> stimulation with C-strain CSFV or an uninfected cell preparation (Mock) and data presented as the mock corrected values. (B) Serum neutralisation titres (SNT) against the CSFV reference strain Alfort 187 were assessed <i>in vitro</i>. (C) Viraemia in was measured by qRT-PCR and expressed as log₁₀ viral RNA copies/µl blood. Data from the UK2000/7.1 and CBR/93 experiments were combined and results expressed as the mean data for each group of pigs and error bars represent SEM.