Mononucleated muscle product cells are active in DNA synthesis.

<p>(A) Following myotube differentiation, cells were transfected with myogenin siRNA. Growth medium was then added to the cells and placed for time lapse microscopy. 64 h after transfection with myogenin siRNA, myotubes cleaved into mononucleated cells (arrows indicate cleaved cells). Cells were then stained with EdU. Cells which derived from the cleavage of myotubes (indicated by arrows) incorporated high levels of EdU. (B) Following myotube differentiation, cells were transfected with myogenin siRNA. Growth medium was then added to the cells. Following various incubation time points of transfected cells in growth medium, 10, 20, 40 and 60 hours, cells were then stained with EdU. The number of EdU-positive cleaved cells was counted in 5 random microscopic fields for each time point (Scale, 200 µm).