Responses to intra-MnPO injection of H3R agonist and sulprostone in w-t and Kv4.2−/− mice.

<p>A. Intra-MnPO injection of H3R agonist (10 µM, 0.2 µl) induced hyperthermia (upper panel) and did not affect motor activity (MA)(lower panel) in w-t and Kv4.2−/− mice (red and black traces, respectively). The CBT response was significantly smaller in Kv4.2−/− mice (two-way repeated measures ANOVA, followed by t-tests for each time point, * P<0.05). B. Intra-MnPO injection of EP1/3 agonist sulprostone (10 µM, 0.2 µL) induced hyperthermia (upper panel) and did not affect motor activity (MA)(lower panel) in w-t and Kv4.2−/− mice. The responses were not different in the w-t and Kv4.2−/− mice (P>0.4 two-way repeated measures ANOVA). C. Intra-MnPO injection of PaTx1(800 nM, 0.2 µL) or PaTx1+H3 agonist (800 nM and 10 µM, 0.2 µL) induced a slight hyperthermia of 0.4 and 0.6°C, respectively, (upper panel) and did not affect motor activity (MA)(lower panel) in w-t and Kv4.2−/− mice. The responses were not different (P>0.2 two-way repeated measures ANOVA). A–C. The line graphs represent averages±S.D. (n = 6 mice) through the 10 h recording period. Experiments were carried out in parallel in 2 groups of 6 mice for each treatment.