<p>Schematic representation of p<i>Tv</i>LUC vector constructs (A); <i>Tv</i>PRAC5′UTR, upstream of the <i>Tv</i>PRAC region and containing spliced leader acceptor site; Tubαβ, intergenic region between alpha and beta tubulin genes; NeoR, neomycin phosphotransferase gene; Tubβα, intergenic region between β and α tubulin genes. Representation of the ribosomal promoter region cloned into p<i>Tv-</i>Luc and associated luminescence in Relative Light Units (RLU) detected in the supernatant 48 h after epimastigote transfection with p<i>TvS</i>rDNA-Luc and p<i>TvL</i>rDNA-Luc (B).</p
A. R4L1pMpGWB structures. R4L1pMpGWB101-195, R4L1pMpGWB201-295, R4L1pMpGWB301-395 and R4L1pMpGWB401-...
Vector maps of the tet-inducible pLEW.TbLC2::BCCP His6/eGFP plasmid generated by cloning the preasse...
<p>The transcriptional start site (TSS), labeled 0, is from NCBI (NM_025910.3). Internal control pla...
<p>(A). <i>T. vivax</i> axenic epimastigotes were transfected with circular or <i>AfeI</i> linearize...
<p>(<b>A</b>) Schematic representation of the SSU rDNA locus after integration of the linearized con...
<p>We used a modular format for the construction of the pTb-AMluc series of vectors that were design...
<p>(A) Schematic illustrations of firefly luciferase (F-Luc) expression vectors for transcription of...
To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol...
<p>(A) The structures of luciferase reporter vectors used are shown. Black and grey boxes at downstr...
Abstract Background Heterologous promoter analysis in Plasmodium ha...
<p>(A) Luciferase reporter constructs with different inserts were transiently transfected in HEK293 ...
International audienceTrypanosoma vivax is one of the most common parasites responsible for animal t...
<p>The initiating viruses for the passage series were derived from the infectious clone, pToto-luc (...
<p>(A) Box and whiskers plots of the relative luciferase expression from pGL3 reporter constructs tr...
<p>(<b>A</b>) Hsu cells were transfected with <i>Renilla</i> luciferase plasmid containing Vago prom...
A. R4L1pMpGWB structures. R4L1pMpGWB101-195, R4L1pMpGWB201-295, R4L1pMpGWB301-395 and R4L1pMpGWB401-...
Vector maps of the tet-inducible pLEW.TbLC2::BCCP His6/eGFP plasmid generated by cloning the preasse...
<p>The transcriptional start site (TSS), labeled 0, is from NCBI (NM_025910.3). Internal control pla...
<p>(A). <i>T. vivax</i> axenic epimastigotes were transfected with circular or <i>AfeI</i> linearize...
<p>(<b>A</b>) Schematic representation of the SSU rDNA locus after integration of the linearized con...
<p>We used a modular format for the construction of the pTb-AMluc series of vectors that were design...
<p>(A) Schematic illustrations of firefly luciferase (F-Luc) expression vectors for transcription of...
To improve transfection efficiency in Trypanosoma cruzi, we developed a new electroporation protocol...
<p>(A) The structures of luciferase reporter vectors used are shown. Black and grey boxes at downstr...
Abstract Background Heterologous promoter analysis in Plasmodium ha...
<p>(A) Luciferase reporter constructs with different inserts were transiently transfected in HEK293 ...
International audienceTrypanosoma vivax is one of the most common parasites responsible for animal t...
<p>The initiating viruses for the passage series were derived from the infectious clone, pToto-luc (...
<p>(A) Box and whiskers plots of the relative luciferase expression from pGL3 reporter constructs tr...
<p>(<b>A</b>) Hsu cells were transfected with <i>Renilla</i> luciferase plasmid containing Vago prom...
A. R4L1pMpGWB structures. R4L1pMpGWB101-195, R4L1pMpGWB201-295, R4L1pMpGWB301-395 and R4L1pMpGWB401-...
Vector maps of the tet-inducible pLEW.TbLC2::BCCP His6/eGFP plasmid generated by cloning the preasse...
<p>The transcriptional start site (TSS), labeled 0, is from NCBI (NM_025910.3). Internal control pla...