FISH and Repeated FISH.

<p>A. FISH of female cells, showing two SpectrumOrange X FISH spots and of a presumed male cell indicated by an arrow, showing one SpectrumOrange X FISH and one SpectrumGreen Y FISH spot; B. Repeated FISH of the female cells and the presumed male cell, showing no SpectrumAqua Y FISH spots in the female cells. In contrary, the male cell shows one SpectrumAqua Y FISH signal on the exact same location as the SpectrumGreen Y FISH spot in image A (indicated by an arrow), indicating this is a true male cell. C. FISH of female cells and of one presumed male cell, indicated by an arrow. D. Repeated FISH of a male cell (C) shows no SpectrumAqua Y FISH spot. The SpectrumGreen Y FISH spot was probably caused by cellular debris or dust particles. The SpectrumOrange X FISH spot of that cell is larger than the other SpectrumOrange spots which may indicate two SpectrumOrange X FISH spots lying very closely to each other.