Gene expression in strains expressing Clp1 proteins carrying a mutant P-loop.

<p>(A) Northern analysis of total RNAs obtained from wild-type and <i>CLP1</i> D161A and <i>CLP1</i> K136A T137A mutant strains following growth in YPD. Probes were as described in the legend of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029139#pone-0029139-g001" target="_blank">figure 1C</a>. (B) qRT-PCR analysis of candidate mRNAs that were identified during gene expression profiling of <i>CLP1</i> D161A and <i>CLP1</i> K136A T137A mutant strains. Transcript levels are presented relative to wild-type <i>CLP1</i>, which was fixed at 100%. Data shown are the mean of three independent biological replicates and error bars indicate standard deviation.