Silencing of Dynamin II expression partially inhibited chemokine endocytosis.

<p>(A) DIH cells were pretreated with dynasore prior to incubation with ¹²⁵I-CXCL1/GRO-α for the specified time points, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029624#s4" target="_blank">Materials and Methods</a>. Dynasore inhibited CXCL1 internalization in DIH cells. * <i>p</i><0.05 dynasore treatment <i>vs</i> control, paired t test. (B) DIH cells were transfected with SiRNA targeted against dynamin II and control SiRNA (ScRNA). At 72 hour post-transfection, endocytosis experiments were performed and cells lysates were analysed by SDS-PAGES and immunoblotted with dynamin II and β-actin antisera. The mean ± SEM from three independent experiments is shown.