Assessment of Tpmt-associated phenotypes in model astroglial cell lines.

<p>Tpmt protein activity was measured in T98 and A172 glioma cell lines. (a) T98 displayed a significantly higher level of protein activity than A172 (p<0.05). (b) As observed in primary astrocyte cultures, low Tpmt protein activity is associated with greater cytotoxicity as determined by the MTT assay; (c) and reflected in IC₅₀ values. A172 had a significantly more sensitive at all TG concentrations and lower IC₅₀ than T98 (p<0.05). (d) The alkaline comet assay was used to compare TG-induced DNA damage. A172 displayed a significantly greater degree of TG-induced DNA strand breaks than T98 (p = 0.02) as measured by mean comet tail moment. Bars represent the means; whiskers represent the standard deviation (±); * p<0.05; ** p<0.005.