Infection of VEFs with CPXV, LV, and TBEV.

<p>Cells grown on coverslips were infected with indicated viruses at a MOI of 0.1, fixed and stained (in green) for CPXV at 17 hours post infection (hpi) (A), LV at 8 hpi (B), and for TBEV at 24 hpi (C). Nuclei were visualized with DAPI staining (blue). To measure virus production, VEFs were infected with CPXV at a MOI of 0.1 (D), LV at a MOI of 60 (E) or TBEV at a MOI of 100 (F) and cultivation media was collected at the indicated time points post infection. Virus progeny production (virus/ml cell culture medium) was determined by titration on Vero E6. Error bars represent standard deviations of the means from one experiment.