Contamination of commercial RT-PCR reagents with murine leukemia virus (MLV).¹

1<p>The reverse transcriptase (RT) enzymes and kits were tested by quantitative protease (<i>pro</i>), polymerase (<i>pol</i>), <i>gag and</i>/or nested <i>pol</i> RT-PCR assays and results shown are number of positive tests out of the total number of replicates. Percentages for replicate testing and copies/reaction (rxn) are in parentheses.</p>2<p>Mouse DNA contamination was detected using mitochondrial DNA (mtDNA) and intracisternal A particle (IAP) PCR qPCR assays.</p>3<p>ND, not done.