Dynasore blocks sterol-sensitive genes regulation in HeLa cells and HMDM.

<p>(A) Kinetics of LDLR expression analyzed by RT-PCR. HeLa cells were grown in LPDS medium for 48 h and further incubated for the indicated times with medium containing either LPDS, 200 µg/ml LDL, or 200 µg/ml LDL with 80 µM dynasore. (B) The expression level of sterol-sensitive genes (LDLR, HMGCoAR and SREBF-2) was quantified after 6 h in HeLa cells grown in LPDS, with 200 µg/ml LDL or 200 µg/ml LDL with 80 µM dynasore, as indicated. (C) The same experiment was performed in HMDM with 50 µg/ml AcLDL. Relative quantification of LDLR, HMGCoAR, and SREBF-2 genes in HeLa cells or HMDM was expressed as fold-variation over control (LPDS/DMSO) after normalization. All CT determinations were made in triplicate.