Analysis of pre-rRNAs intermediates in HeLa and HEK293 Flp-In T-Rex cells transiently transfected with a siRNA targeting the FTSJ3 mRNA.

<p>(<b>A</b>) Western blot analysis to determine FTSJ3 levels 72 h following siRNA transfection. (<b>B</b>) Northern blot using probe P3 complementary to ITS1 upstream site 2b. (<b>C</b>) Graphs representing the ratio between the 34S and 47S pre-rRNA of the Northern blots shown in B. Bands were quantified by using ImageQuant software. Control cells were transfected with siRNA targeting the GFP mRNA.