C3d deposition in chronic hypoxia-induced PH in mice.

<p>(A–D) Lungs from normoxic and hypoxic C57Bl/6J mice were stained with α-C3d and α-SMA antibody and counterstained with DAPI to detect nuclei(n = 4). (E) Quantification of C3d staining in WT vs. C3−/− mice in normoxia and hypoxia. Bars represent the mean ± SD (n = 4). *<i>P</i><0.05. (F) Representative Western blot for C3d in normoxic vs. hypoxic C57Bl/6J mice. (G) Quantification of Western blots for C3d in normoxic vs. hypoxic mice. Bars represent the mean ± SD (n = 4). *<i>P</i><0.05. (H) C5a was immunoprecipitated from lung or plasma of normoxic and hypoxic C57Bl/6J mice and analyzed by Western blot (rC5a = recombinant mouse C5a).