MTDH regulates LPS-induced NF-κB activation and cytokine production.

<p>(<b>A</b>) Protein expression levels were determined by Western-blot assays. Protein extracts were prepared from the prp-control and prp-MTDH MDA-MB-231 cells treated without or with LPS (100 ng/ml) for 1 hour. (<b>B, C</b>) The mRNA levels of IL-8 (<b>B</b>) and MMP-9 (<b>C</b>) were measured by Real Time RT-PCR. Total RNA was extracted from cells in with or without of LPS (100 ng/ml) for 24 hours. β-actin was used as an endogenous control. Each experiment was carried out three times independently.