MCH-GFP perikarya and axons and TH axons at E14–15.

<p>Photomicrographs of the GFP labeling on horizontal sections of a E14 (A–C) and E15 (D) MCH-GFP embryos. (A–C) At E14, many MCH-GFP perikarya (A) are observed in the tuberal hypothalamus and some fibers take a rostral direction toward the telencephalon. On the ventrally adjacent section (B), MCH-AS labels perikarya, but axons are not detected. (C) On the section dorsally adjacent to A, a tyrosine hydroxylase (TH)-AS intensely labeled mesostriatal axons in the medial forebrain bundle (mfb). (D) At E15, rostrally directed axons are very abundant in the mfb. Scale bar: A, D = 200 µm; B, C = 500 µm. ANT: anterior level, hypothalamus; MAM: mammillary level hypothalamus; V3: third ventricle.