Day-4 DCs derived from EOC patients, stimulated potent and specific autologous ovarian tumor-reactive T cell responses <i>in vitro</i>.

<p>Patients' T cells were cocultured with autologous Day-2, Day-4 or Day-7 mature DCs previously pulsed with SKOV3 UVBL or FTL for 2 weeks and then interrogated for reactivity against tumor antigens. The IFN-γ responses of patients' T cells previously stimulated with UVBL-pulsed DCs (closed squares or black bars), FTL-pulsed DCs (closed circles or white bars) or unpulsed mature DCs (closed triangles or grey bars) are shown here, (A) For patients 1 and 2 who were HLA-A2⁻, the T cells were tested in the presence of autologous DCs pulsed with SKOV3 FTL, autologous unpulsed DCs, or media (T cells only). (B) For patient 3, who was HLA-A2⁺, the T cells were also tested against live HLA-A2⁺ SKOV3 cells, HLA-A2⁺ T2 pulsed with HER-2/neu 369 or 689 peptides, autologous DCs pulsed with HLA-A2⁻ SKOV3 FTL, autologous unpulsed DCs, or media (T cells only). The results are the means of the number of IFN-γ spots per 10⁶ T cells ± standard error. The asterisks indicate those columns differing significantly (<i>P</i> value = 0.001; Student's paired <i>t</i> test) from the media (i.e. T cells only) controls.