The effect of initial PCR efficiency (E<sub>i</sub> = 100% and 20%) on PCR product yield.
- Chih-Hui Lin (337569)
- Yu-Chieh Chen (126845)
- Tzu-Ming Pan (156183)
Publication date
February 2013
DOIAbstract
a<p>A<sub>n</sub> = A<sub>0</sub> = A = initial copy number of DNA template (Ci).</p>b<p>B<sub>n</sub> = A×E+B<sub>n−1</sub>×1 (B<sub>0</sub> = 0).</p>c<p>C<sub>n</sub> = B<sub>n−1</sub>×1+C<sub>n−1</sub>×2 (C<sub>0</sub> = C<sub>1</sub> = 0).</p>d<p>D<sub>n</sub> = A<sub>n</sub>+B<sub>n</sub>+C<sub>n</sub>.</p>e<p>Bias = (compromised yield−theoretical yield)/theoretical yield×100%.</p
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