Precision: Intra-assay and inter-observer variation.

<p><b>A&B)</b> Two sets of assays were performed by CBCA or CBFCA, set 1 includes 12 assays (gray diamonds) and set 2 includes 15 assays (black squares). Intra-assay coefficient of variation (CV) in CBCA was higher than in CBFCA in both sets (30.1 and 17.3% vs 4.7 and 4.8%). <b>D&C)</b> Three of these assays were counted by three different observers/operators using microscopy or flow cytometry. The inter-observer CV in CBCA was higher than in CBFCA (30.1 vs 3.6%). Data are shown as means±SD of three independent assays counted in triplicate.