Rescue of myofibril organization defect in smyd1b knockdown embryos by expression of Smyd1b_tv1-EGFP or Smyd1b_tv2-EGFP fusion protein.

<p><b>A.</b> DNA constructs encoding Smyd1b_tv1-EGFP, or Smyd1b_tv2-EGFP fusion proteins or EGFP control were generated and injected into zebrafish embryos. <b>B and C.</b> Myofibers expressing Smyd1_tv1-EGFP (B) or Smyd1_tv2-EGFP (C) was directly observed by GFP. <b>D and E.</b> Myosin thick filaments organization was determined by F59 antibody staining in Smyd1_tv1-EGFP (D) or Smyd1_tv2-EGFP (E) co-injected embryos. <b>F and G.</b> Double staining shows the colocalization of normal fibers with Smyd1_tv1-EGFP (F) or Smyd1_tv2-EGFP (G) expression. Scale bars: 20 µm.