Availability of gal-1 influences HTR-8/SVneo cell invasion.

<p>Cells were incubated with 1 and 5 µg/ml of non-immune IgG or anti-gal-1 antibodies (A), 1 µg/ml of CS-gal-1 or Ox-gal-1 alone (B) or in combination with 0.1 M lactose (C). Cells on the underside of the filters and the occupied pores were counted after 24 h of culture. The data are expressed as the percentage of pores occupied in the treatment compared to the control value, with data given as mean ± S.E.M. For treatments with CS-gal-1 and Ox-gal-1 data were normalized to take into account the increase in cell number.The presence of function-blocking anti-gal-1 (A, at 1 and 5 µg/ml) significantly reduced invasion with no difference between the two concentrations (<i>P</i><0.001, four experiments, in duplicate for 1 µg/ml; <i>P</i><0.001, eight experiments in duplicate for 5 µg/ml of anti-gal-1 IgG). The addition of gal-1 significantly stimulated cell invasion, which was considerably lower for Ox-gal-1 than for CS-gal-1 at 1 µg/ml (B, <i>P</i><0.001 for CS-gal-1, <i>P</i><0.001 for Ox-gal-1; three experiments in duplicate for each molecular form of gal-1). This stimulatory activity was abolished in the presence of 0.1 M lactose for both forms of recombinant gal-1 (C). Squares above the bars represent typical fields of the invasion insert.