Rosiglitazone inhibited TGF-β1-induced collagen type I expression in ADPKD cyst-lining epithelial cells through PPARγ.

<p>(A) Cells were incubated with GW9662 (1 µmol/L) and cultured for 48 h. Then cells were pretreated with 10 µmol/L rosiglitazone for 1 h and incubated with TGF-β1 (5 ng/mL) for 24 h. (B) Cells were transfected with a PPARγ siRNA for 48 h, then pretreated with 10 µmol/L rosiglitazone for 1 h and incubated with TGF-β1(5 ng/mL) for 24 h. (C) PPARγ mRNA was decreased to 36% using real-time RT–PCR in PPARγ siRNA-transfected ADPKD cyst-lining epithelial cells. The results were representative of three independent experiments. *P<0.05 vs. control; #P<0.05 vs. TGF-β1 alone Δ P<0.05 vs. rosiglitazone + TGF-β1.